Nitrate Reductase Activity As An Indication of Molybdenum Level

Nitrate reductase activity determined in the leaf fragments of citrus plants, grown in gradually decreasing amounts of Mo in the solution culture, was used for the estimation of molybdenum level of the leaves.

The sensitivity of the assay was tested in comparison with the chemical analysis by means of dithiol or the bioassay with A. niger, and was found satisfactory.

The rate of increase in nitrate reductase activity in response to Mo infiltration into the leaf fragments was negatively associated with the Mo level of the substrate and the leaf tissue.

And it is suggested as a quick test for the determination of Mo requirement of the corresponding trees.


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